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Almost all lin-32 mutants failed to express inx-1 ( Fig 2C , S3 Table ). INX-1 is the only known electrical synapse component of AIB interneurons [ high waisted utility trousers Black Julius jtZlE
]. To examine how AIB electrical synapses contributed to the optimization of avoidance behaviors, we analyzed the degrees of the behavioral defects of the inx-1 null mutants. Compared to the wild type animals ( Alexander Wang Woman Silver And Goldtone Chain Choker Silver Size Alexander Wang UC05KD5Eca
), the inx-1 mutants exhibited significantly reduced omega turns in 2 M, 4 M and 6 M sorbitol ( Fig 5A ) (p < 0.0001, ANOVA followed by the Tukey's post hoc tests). The defects in inx-1 seemed to be slightly weaker than those of lin-32 , but the differences were not significant between inx-1 mutants and lin-32 mutants or lin-32 inx-1 double mutants ( Fig 5B ) (p > 0.05, respectively. ANOVA followed by the Tukey's post hoc tests). These behavioral data suggest inx-1 is downstream of lin-32 .

Fig 5. AIB electrical synapses are required for the optimization of probability of omega turn.

(A) inx-1 mutants show impaired avoidance behaviors independent on the sorbitol concentration. n = 33,10,35,10,10. (B) The frequency of avoidance behaviors toward 2 M sorbitol. inx-1 mutants show reduced omega turns compared to the wild type animals. The inx-1 phenotypes were not different from those of lin-32 mutants and lin-32 inx-1 double mutants. n = 4,4,4,12. (C) Expression of inx-1 cDNA driven by its own promoter ( inx-1; Pinx-1 :: INX-1 ) and the AIB lineage-selectively promoter ( inx-1 ; Punc-130 :: INX-1 ) fully rescues osmotic avoidance defects in response to 2 M sorbitol. n = 21,21,22,13. (D) Expression of Punc-130 :: INX-1 cDNA fully rescues osmotic avoidance defects in lin-32 mutants in response to 2 M sorbitol. n = 21,21,13. (E) AIB::gfp::TeTx transgenic animals can adjust probability of omega turns depending on the sorbitol drop concentration. n = 8,8,9,9,9. n = plate (cohort) of approximately 10–20 animals. The data are presented as the mean ± SEM.

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We performed AIB-specific INX-1 rescue experiments to verify that inx-1 acted in AIB. The results showed that the expression of INX-1 cDNA under Punc-130 , could fully rescue the defect, as could the authentic inx-1 promoter ( Fig 5C ). In addition, INX-1 cDNA expression under the control of Punc-130 also fully rescued the defects in the lin-32 mutants ( Modal Scarf Grey Tribe by VIDA VIDA kGHmAla
). In both cases, there were significant differences relative to each single mutant (p < 0.001, respectively. ANOVA followed by the Tukey's post hoc tests), and no significant differences relative to the wild type animals (p > 0.05, respectively. ANOVA followed by the Tukey's post hoc tests).

“TELUS’ connected car strategy builds upon our successful Internet of Things (IoT) business which includes developing innovative solutions for both the business and consumer markets across Canada,” said Rich Osborn, Managing Partner, TELUS Ventures. “Through venture investments in emerging technology leaders like Mojio, we not only bring better solutions to market more quickly for our customers and drive better connections, but also help propel the Connected Car market in Canada and internationally with complementary telecom carriers around the world.” Mojio is connecting cars all over the world, from a 1996 Audi A4 to a brand new 2018 Volvo V60, helping drivers and their families stay coordinated, while keeping their vehicles safe, and cutting down on costly, time-consuming issues associated with car ownership. Common use cases include:

In addition to delivering frontline services for consumers through their mobile networks, Mojio has already collected over 6 billion miles of driving data. Mojio uses machine learning to generate insights that will shape the future of mobility, making the driving experience smarter, safer and less expensive.

About Mojio Open, scalable and hardware agnostic, Mojio is the cloud platform of choice for the deployment of secure connected car apps and services. Mojio’s platform delivers a smarter, safer and more convenient driving and vehicle ownership experience to subscribers of major network operators, including Bell Canada, Deutsche Telekom, Rogers Communications, T-Mobile and TELUS. With real-world data gathered from more than 6 billion miles of driving, Mojio provides a big data analytics framework that uses machine learning to generate actionable insights and unlock new revenue streams for companies throughout the automotive value chain. Founded in 2012, Mojio has growing teams in Vancouver, Palo Alto and Sofia, and some of the world’s biggest brands as customers, partners and investors, Mojio is a career-accelerator for driven tech professionals looking to shape the future of mobility. To learn more about joining our team, please visit Mojio’s careers page .

About Iris Capital Iris Capital is a European venture capital funds’ manager specialized in the digital economy. It fuels entrepreneurs at various stages of maturity, from early-stage to growth equity. With its deep sector specialization and extensive experience acquired over 30+ years, as well as the backing of its corporate sponsors, Iris Capital provides active support to its portfolio companies, through its presence in Paris, Berlin, San Francisco, Tel Aviv, Tokyo and Dubai.

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